CpG Analyzer, a Windows-based utility program for investigation of DNA methylation
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چکیده
منابع مشابه
CpG analyzer, a Windows-based utility program for investigation of DNA methylation.
656 BioTechniques Vol. 39, No. 5 (2005) There is substantial evidence that changes in DNA methylation occur during preneoplasia, including both global changes in DNA methylation and changes in CpG dinucleotide methylation sites in specific genes (1). Aberrant DNA methylation within CpG islands is one of the earliest and more common alterations in human malignancies (2). Cytosine methylation in ...
متن کاملCpG methylation patterns of human mitochondrial DNA
The epigenetic modification of mitochondrial DNA (mtDNA) is still in controversy. To clarify this point, we applied the gold standard method for DNA methylation, bisulfite pyrosequencing, to examine human mtDNA methylation status. Before bisulfite conversion, BamHI was used to digest DNA to open the loop of mtDNA. The results demonstrated that the linear mtDNA had significantly higher bisulfite...
متن کاملDNA Methyltransferases and Methylation of CpG islands
DNA methylation is a critical mechanism of epigenetic modification involved in gene expression programming, that can promote the development of several cancers, including breast cancer. The methylation of CpG islands by DNA methyltransferases is reversible and has been shown to modify the transcriptional activity of key proliferation genes or transcription factors involved in suppression or pro...
متن کاملSpreadsheet-based program for the analysis of DNA methylation.
Methylation of DNA in CpG dense regions of gene promoters (CpG islands) is important for transcriptional inactivation of selective genes in normal and neoplastic cells. Here, we present a spreadsheet-based program adapted from Microsoft Excel that is useful for identifying CpG islands and for assisting in the laboratory analysis of DNA methylation of these regions. Upon execution of the program...
متن کاملMIRA-seq for DNA methylation analysis of CpG islands
AIM To develop a reliable method for whole genome analysis of DNA methylation. MATERIALS & METHODS Genome-scale analysis of DNA methylation includes affinity-based approaches such as enrichment using methyl-CpG-binding proteins. One of these methods, the methylated-CpG island recovery assay (MIRA), is based on the high affinity of the MBD2b-MBD3L1 complex for CpG-methylated DNA. Here we provi...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2005
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000112053